rabbit anti cyclin d1 polyclonal ab  (Santa Cruz Biotechnology)

Journal: Tumor Biology

Article Title: Identification of interleukin-16 production on tumor aggravation in hepatocellular carcinoma by a proteomics approach

doi: 10.3233/tub-211507

Figure Lengend Snippet: Fig. 4. Western blot band intensities of (a) ERK1/2 protein and phosphorylated ERK1/2 protein and (b) cyclin D1 in HCC cell lines. HCC cells were treated with IL-16 for 48 h. Total ERK1/2 increased significantly in HepG2 and Huh7 cells in a dose-dependent manner. Data indicate the mean ± SEM. ERK1/2 and phosphorylated ERK1/2 data are representative of six independent experiments. Cyclin D1 data are representative of five (HepG2) and six (Huh7) independent experiments. -Tubulin was used as a loading control and to normalize protein intensities. ERK, extracellular signal-regulated protein kinase.

Article Snippet: In addition to anti-human IL-16 Ab, the following Abs were used: rabbit anti-ERK1/2 polyclonal Ab, rabbit anti-phospho-ERK1/2 polyclonal Ab, and rabbit anti-cyclin D1 polyclonal Ab (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Western Blot, Control

Journal: Biomedicines

Article Title: Tussilagone Reduces Tumorigenesis by Diminishing Inflammation in Experimental Colitis-Associated Colon Cancer

doi: 10.3390/biomedicines8040086

Figure Lengend Snippet: Administration of tussilagone inhibits the β-catenin expression in the colon tissues. ( a ) The protein samples in the cytosol or nuclear fraction from the colon tissues were separated by SDS-PAGE. β-catenin was detected by Western blot and visualized by chemiluminescence. Photos are representative images. ( b ) The quantitative data were normalized by internal control (β-actin for cytosol; lamin-B for nuclear fraction) and further expressed as folds, presented as the comparison with the amount relative to the AOM/DSS-treated mice. ( c ) The immunohistochemical analysis of nuclear β-catenin expression in the colon tissues (upper, ×100 magnification; lower, ×400 magnification; bar = 100 µm). ( d ) The percentage of nuclear β-catenin positive cells in the colon tissues was calculated as described in Methods. ( e ) The protein samples of the whole-cell lysates from the colon tissues were separated by SDS-PAGE. Cyclin D1 was detected by Western blot and visualized by chemiluminescence. Photos are representative images. ( f ) The quantitative data were normalized by internal control, β-actin and further expressed as folds, presented as the comparison with the amount relative to the AOM/DSS-treated mice. The results are expressed as the mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the AOM/DSS-treated mice.

Article Snippet: We did Western blot and immunohistochemistry analyses using the following antibodies (ab): anti-nuclear factor erythroid 2-related factor 2 (Nrf2) rabbit polyclonal ab, anti-heme oxygenase (HO)-1 mouse monoclonal ab, anti-IL-6 goat polyclonal ab, anti-TNF-α mouse monoclonal ab, anti-proliferating cell nuclear antigen (PCNA) mouse monoclonal ab (all Santa Cruz Biotechnology, Santa Cruz, CA), anti-cleaved-Poly (ADP-ribose) polymerase (PARP) rabbit polyclonal ab, anti-NF-κB p65 rabbit polyclonal ab, anti-β-catenin rabbit polyclonal ab, anti-cyclin D1 rabbit polyclonal ab (all Cell Signaling Technology, Danvers, USA), anti-inducible nitric oxide synthase (iNOS) rabbit polyclonal ab (BD Bioscience, Becton Dickinson, USA) and anti-cyclooxygenase (COX)-2 rabbit polyclonal ab (Cayman Chemical, Ann Arbor, MI, USA).

Techniques: Expressing, SDS Page, Western Blot, Immunohistochemical staining

Journal: Oncotarget

Article Title: RASSF8 regulates progression of cutaneous melanoma through nuclear factor-κb.

doi: 10.18632/oncotarget.5030

Figure Lengend Snippet: Figure 3: Overexpression of RASSF8 induced cell apoptosis by regulating P53-P21 pathway. A. Cell cycle G1/S stage was arrested when RASSF8 was overexpressed. B. Apoptosis was induced when RASSF8 was overexpressed in melanoma cell. C. Caspase activity was increased when RASSF8 was overexpressed in melanoma cell. D. The expression of P53 and P21 were induced by RASSF8 whereas Cyclin D1 was reduced by RASSF8. E. Apoptosis array assay showed that P21 and TRAIL R2/DR5 were induced in cell apoptosis caused by RASSF8.

Article Snippet: Membranes were immunoblotted overnight with primary mouse monoclonal anti-RASSF8 Ab (1:1000, Abcam, Cambridge, MA ,Cat.# ab56921), anti-P53 (BD Biosciences, Cat.# 554294) and anti-P21 (BD Biosciences, Cat.# 556431), mouse monoclonal anti-P65 Ab (Santa Cruz, Cat.# sc-8008) and rabbit polyclonal anti-Cyclin D1 Ab (1:1000, Santa Cruz, Cat.# sc-753), rabbit polyclonal anti-P50 Ab (1:1000, Cell Signaling, Danvers, MA, Cat.# 3035) and rabbit anti-IκBα Ab, anti-p-65 (1:1000, Cell Signaling, Danvers, MA, Cat.# 9936 and 3033).

Techniques: Over Expression, Activity Assay, Expressing